In vitro culture of frozen and thawed mouse ova.
نویسنده
چکیده
منابع مشابه
The Effects of Plla Nanofiber Scaffold on Proliferation of Frozen-Thawed Neonate Mouse Spermatogonial Stem Cells
Purpose: To investigate of the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on proliferation of frozen-thawed neonate mouse spermatogonial stem cells.Materials and Methods: Spermatogonial cells were isolated from neonatal 3-6-day-old NMRI mice testes by two steps enzymatic digestion and differential plating. The isolated spermatogonial cells were divided into four culture groups: 1...
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Background: This study was set up to evaluate the effect of ovarian tissue slow freezing on in vitro growth and pattern of maturation genes expression in mouse preantral follicles encapsulated within alginate hydrogel. Materials and Methods: Ovaries of 12-14 days old female NMRI mice were randomly allocated into control and slow freezing groups. In slow freezing group, ovaries were equilibrated...
متن کاملP-128: Fibroblast Growth Factor Improves Oocyte Maturation in Vitrified-Thawed Mouse Follicles
Background: The fibroblast growth factors (FGF-4) are a group of heparin-binding single chain polypeptides that play a pivotal role in development, cell growth, tissue repairing and transformation. The aim of this study is to improve development and maturation outcome of vitrified mouse pre-antral follicle by adding of fibroblast growth factor (FGF) into medium culture. Materials and Methods: P...
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The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...
متن کاملThe effects of poly L-lactic acid nanofiber scaffold on mouse spermatogonial stem cell culture
INTRODUCTION A 3D-nanofiber scaffold acts in a similar way to the extracellular matrix (ECM)/basement membrane that enhances the proliferation and self-renewal of stem cells. The goal of the present study was to investigate the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on frozen-thawed neonate mouse spermatogonial stem cells (SSCs) and testis tissues. METHODS The isolated sper...
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ورودعنوان ژورنال:
- The Japanese journal of veterinary research
دوره 28 1-2 شماره
صفحات -
تاریخ انتشار 1980